General Cell Based Assays
Bioassay Systems EnzyChrom™ HDL and LDL/VLDL Assay Kit. For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism.
For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism. Key Features: Sensitive and accurate. Requires only 20 µL serum sample. Detection limit of 5 mg/dL, linearity up to 300 mg/dL cholesterol in 96-well plate assay. Convenient. Room temperature assay. No 37°C heater is needed. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD340nm. Samples: Serum. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 5 mg/dL.
Bioassay Systems EnzyChrom™ Lactose Assay Kit. For quantitative determination of lactose and evaluation of drug effects on lactose metabolism.
For quantitative determination of lactose and evaluation of drug effects on lactose metabolism. Key Features: Sensitive. Use as little as 20 µL samples. Linear detection range in 96-well plate: 17 to 2000 µM lactose for colorimetric assays and 6 to 100 µM for fluorimetric assays. Convenient and high-throughput. Robust and homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological (milk), food and agriculture etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 6 µM.
Bioassay Systems EnzyChrom™ Adipolysis Assay Kit. For quantitative assay of adipolysis and evaluation of drug effects on adipolysis.
For quantitative assay of adipolysis and evaluation of drug effects on adipolysis. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 0.92 to 100 µg/mL (10 to 1000 µM) glycerol for colorimetric assays and 0.2 to 5 µg/mL for fluorimetric assays. Rapid and convenient.The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Robust and amenable to HTS assays. Potential interference by testing drugs is greatly reduced at 570nm. Compatible with culture media containing phenol red. Assays can be performed in 96 or 384-well plates. Method: OD570nm, or FL530/585nm. Samples: Cell culture media. Species: All. Procedure: Assay takes 20 min. Kit size: 200 tests. Detection limit: 0.92 µg/mL.
Bioassay Systems EnzyChrom™ a-Amylase Assay Kit. For quantitative determination of a-amylase activity.
For quantitative determination of α-amylase enzyme activity. Key Features: Sensitive and accurate. Linear detection range 0.3 to 50 U/L α-amylase in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, incubation for 15 min, followed by the detection reagent and a 20-min incubation and reading the optical density at 585 nm. Method: OD585nm. Samples: Blood, saliva, urine, agriculture etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.3 U/L.
Bioassay Systems EnzyChrom™ Creatine Kinase Assay Kit. For quantitative determination of creatine kinase activity and evaluation of drug effects on CK activity.
For quantitative determination of creatine kinase (CK) activity and evaluation of drug effects on CK activity. Key Features: Sensitive and accurate. Detection range: 5 to 300 U/L creatine kinase in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at 20 min and 40 min at room temperature or 37°C. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD340nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 5 U/L.
Bioassay Systems QuantiChrom™ Magnesium Assay Kit. For quantitative determination of Mg2+ and evaluation of drug effects on magnesium metabolism.
For quantitative determination of Mg2+ and evaluation of drug effects on magnesium metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL sample. Linear detection range 0.1 mg/dL (41 µM) to 3 mg/dL (1.2 mM) Mg2+ in 96-well plate assay. Simple and high-throughput. The procedure involves addition of two reagents and measuring OD500nm. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. Assays can be directly performed in serum and urine samples. Method: OD500nm. Samples: Biological, food and beverage. Species: All. Procedure: Assay takes 10 min. Kit size: 250 tests. Detection limit: 0.1 mg/dL (41 µM).
Bioassay Systems EnzyChrom™ AF HDL and LDL/VLDL Assay Kit. For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism.
For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism. Key Features: Sensitive and accurate. Linear detection range in 96-well plate: 1 to 100 mg/dL cholesterol for colorimetric assays and 0.2 to 10 mg/dL for fluorimetric assays. Convenient. Room temperature assay. No 37°C heater is needed. Method: OD570nm, or FL530/585nm. Samples: Serum. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: OD, FL: 1, 0.2 mg/dL.
Bioassay Systems EnzyChrom™ Cholesterol Assay Kit. For quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism.
For quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism. Key Features: Sensitive and accurate. Detection limit of 5 mg/dL, linearity up to 300 mg/dL cholesterol in 96-well plate assay. Convenient. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD340nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 5 mg/dL.
Bioassay Systems EnzyChrom™ Aspartate Transaminase Assay Kit. For quantitative determination of aspartate transaminase (AST) activity and evaluation of drug effects on AST activity.
For quantitative determination of aspartate transaminase (AST) activity and evaluation of drug effects on AST activity. Key Features: Sensitive. Linear detection range: 2-100 U/L. Simple and convenient. This simple, convenient assay can be carried out in a microplate or a cuvette and takes only 10 min. Method: OD340nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 2 U/L.
Bioassay Systems EnzyChrom™ L-Lactate Assay Kit. For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism.
For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM L-Lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM L-Lactate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, cell culture media etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.05 mM.
Cell Biolabs Inc CytoSelect™ 48-Well Cell Contraction Assay Kit (Floating Matrix Model), 48 assays
Several culture models exist to study fibroblasts' ability of to reorganize and contract collagen matrices in vitro. In the floating matrix contraction model, a freshly polymerized collagen matrix containing cells is released from the culture dish and allowed to float in culture medium, and contraction occurs in the absence of external mechanical load and without appearance of stress fibers in the cells. The CytoSelect™ 48-Well Cell Contraction Assay Kit provides a simple system to assess cell contractivity in vitro and screen cell contraction mediators using the floating matrix model. The kit's proprietary Cell Contraction Plate eliminates the matrix releasing step of the conventional contraction assay, providing a faster, higher-throughput method to assess cell contraction. Each kit provides sufficient quantities to perform up to 48 assays in a 48-well plate.
Epigentek Inc Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Fluorometric) (96 assays)
The Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Fluorometric) is a complete set of optimized buffers and reagents for measuring activity/inhibition of total cytosine oxygenase TET enzymes in nuclear extracts or purified TET isoforms (TETs 1-3) from a broad range of species such as mammals, plants, fungi, and bacteria, and in a variety of forms including, but not limited to cultured cells and fresh and frozen tissues.
Cell Biolabs Inc CytoSelect™ 24-Well Cell Contraction Assay Kit (Floating Matrix Model), 24 assays
Several culture models exist to study fibroblasts' ability of to reorganize and contract collagen matrices in vitro. In the floating matrix contraction model, a freshly polymerized collagen matrix containing cells is released from the culture dish and allowed to float in culture medium, and contraction occurs in the absence of external mechanical load and without appearance of stress fibers in the cells. The CytoSelect™ 24-Well Cell Contraction Assay Kit provides a simple system to assess cell contractivity in vitro and screen cell contraction mediators using the floating matrix model. The kit's proprietary Cell Contraction Plate eliminates the matrix releasing step of the conventional contraction assay, providing a faster, higher-throughput method to assess cell contraction. Each kit provides sufficient quantities to perform up to 24 assays in a 24-well plate.
Cell Biolabs Inc Glutamate Assay Kit (Fluorometric), 200 assays
Excess glutamate levels in the brain can cause cell injury and death, leading to neurological diseases. Cell Biolabs' Glutamate Assay Kit is a quantitative, fluorometric assay that uses glutamate specific enzymes to generate hydrogen peroxide. Glutamate levels as low as 300 nM can be detected in serum, plasma, urine, cell lysates, and cell culture supernatants. Each kit provides sufficient reagents for 200 assays including standards and unknown samples.
Cell Biolabs Inc CytoSelect™ 96-Well Cell Migration Assay Kit (5 µm, Fluorometric), 5x96 assays
Chemotaxis, a type of cell migration, is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. The initial response of a cell to a migration-promoting agent is to polarize and extend protrusions in the direction of the attractant; these protrusions can consist of large, broad lamellipodia or spike-like filopodia that are driven by actin polymerization and can be stabilized by extracellular matrix (ECM) adhesion or cell-cell interactions. The CytoSelect™ Cell Migration Assay Kit utilizes polycarbonate membrane inserts (8 µm pore size) to assay the migratory properties of cells. The kit contains 5x96 polycarbonate membrane inserts with 5 um pores in each of five 96-well plates, through which migratory cells are able to pass. Cells are quantified using the provided fluorometric dye. The 5 µm pore size is optimal for measuring the migration of monocytes and macrophages.