General Cell Based Assays
Cayman Chemical Bilirubin Assay DMSO, 5 mL
Bilirubin assay DMSO has been tested and formulated to work exclusively with Cayman’s Total and Direct Bilirubin Colorimetric Assay Kit (Item No. 701720).
Revvity Health Sciences Inc HTRF Human Phospho-MKK4 (Ser257) Detection Kit, 500 Assay Points
HTRF Human Phospho-MKK4 (Ser257) Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-ERK (Thr202/Tyr204) Advanced Detection Kit, 500 Assay Points
HTRF Human & Mouse Phospho-ERK (Thr202/Tyr204) Advanced Detection Kit, 500 Assay Points
Revvity Health Sciences Inc Terbium SureFire Ultra Human & Mouse Phospho-CREB (Ser133) Detection Kit, 50,000 Assay Points
Terbium SureFire Ultra Human & Mouse Phospho-CREB (Ser133) Detection Kit, 50,000 Assay Points
Eurofins Discoverx cAMP Hunter™ Human VIPR1 Gs/Gq Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human VIPR1 Gs/Gq Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure VIPR1 (GPCR) activity via increased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Revvity Health Sciences Inc HTRF Human Phospho-MKK4 (Ser257) Detection Kit, 10,000 Assay Points
HTRF Human Phospho-MKK4 (Ser257) Detection Kit, 10,000 Assay Points
Cayman Chemical DuP-697 Assay Reagent, CAS: 88149-94-4, 1 ea
DuP-697 Assay Reagent, CAS: 88149-94-4, 1 ea
Cayman Chemical CelBased asy NBD CholSte 500uL
A DYRK1A inhibitor (IC50 0.4 nM) selective for DYRK1A over DYRK2 Clk1 Clk2 Clk3 Cdk2 and GSK3B (IC50s 19 7.1 9.4 54 100 and 94 nM respectively) but does inhibit DYRK1B (IC50 2.7 nM) inhibits DYRK1A-induced increases in tau phosphorylation in a cell-based assay (IC50 590 nM)
Eurofins Discoverx cAMP Hunter eXpress MC3R CHO-K1 GPCR Assay, 2-Plate Kit. (95-0045E2CP2M)
The cAMP Hunter MC3R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. α-melanocyte stimulating hormone) based activation of MC3R GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type MC3R, a naturally Gs coupled GPCR. Upon activation of MC3R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Molecular Devices cAMP Fluorescent Assay Kit, 384-Well, Bulk, 60 μL
The CatchPoint cAMP Fluorescent Assay Kit is a high-throughput competitive immunoassay for quantifying 3',5'-cyclic adenosine monophosphate (cAMP) and measuring adenylate cyclase activity in biochemical and cell-based assays. The 384-well bulk format supplies reagents for 20 plates (7,680 assay points) and is optimized for fluorescence intensity detection with long signal stability.
- 384-well bulk format, sufficient for 20 plates (7,680 wells)
- Reaction volume 60 μL per well, 50 μL supported for some HTS systems
- Fluorescence intensity detection using Stoplight Red substrate
- Minimum detectable concentration 0.1 nM (6 fmol/well), EC50 approximately 3.4 nM
- Readings stable from 10 minutes up to 24 hours after substrate addition
- Store components at 4°C; kit components stable for six months when stored properly
Cell Biolabs Inc NAD+/NADH Assay Kit (Colorimetric), 100 assays
Cell Biolabs’ NAD+/NADH Assay Kit (Colorimetric) is a simple assay that measures NAD+ and NADH present in biological samples such as cell lysates or tissue extracts in a 96-well microtiter plate format. The kit is specific for NAD+, NADH, and their ratio. The kit will not detect NADP+ or NADPH. The total NAD+/NADH concentrations of unknown samples are determined by comparison with a known NAD+ standard. Determination of both NAD+ and NADH requires two separate samples for quantification. NAD+ and NADH do not need to be purified from samples, but rather can be extracted individually with a simple acid or base treatment prior to performing the assay. The kit has a detection sensitivity limit of approximately 4 nM NAD+. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, NAD+ standards and unknown samples.
Eurofins Discoverx PathHunter eXpress GPR32 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0321E2ACP2L)
The PathHunter GPR32 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR32 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR32 and EA-tagged β-Arrestin-2. Activation of GPR32 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress P2RY6 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0500E2CP19M)
The PathHunter P2RY6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. UTP) based activation of P2RY6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged P2RY6 and EA-tagged β-Arrestin-2. Activation of P2RY6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress NMBR Total Internalization Assay, 2pl Kit. (93-0939E3CP5M)
The PathHunter NMBR Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of NMBR via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged NMBR and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the NMBR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.